The Periplasmic Cavity of LacY Mutant Cys154→Gly: How Open Is Open?
نویسندگان
چکیده
منابع مشابه
Crystal structure of a LacY-nanobody complex in a periplasmic-open conformation.
The lactose permease of Escherichia coli (LacY), a dynamic polytopic membrane protein, catalyzes galactoside-H+ symport and operates by an alternating access mechanism that exhibits multiple conformations, the distribution of which is altered by sugar binding. We have developed single-domain camelid nanobodies (Nbs) against a mutant in an outward (periplasmic)-open conformation to stabilize thi...
متن کاملResidues gating the periplasmic pathway of LacY.
X-ray crystal structures of LacY (lactose permease of Escherichia coli) exhibit a large cytoplasmic cavity containing the residues involved in sugar binding and H(+) translocation at the apex and a tightly packed side facing the periplasm. However, biochemical and biophysical evidence provide a strong indication that a hydrophilic pathway opens on the external surface of LacY with closing of th...
متن کاملSingle-molecule FRET reveals sugar-induced conformational dynamics in LacY.
The N- and C-terminal six-helix bundles of lactose permease (LacY) form a large internal cavity open on the cytoplasmic side and closed on the periplasmic side with a single sugar-binding site at the apex of the cavity near the middle of the molecule. During sugar/H(+) symport, an outward-facing cavity is thought to open with closing of the inward-facing cavity so that the sugar-binding site is...
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Computer platforms provide an integrated architecture of hardware and software standards as a basis for developing complementary assets. The most successful platforms were owned by proprietary sponsors that controlled platform evolution and appropriated associated rewards. Responding to the Internet and open source systems, three traditional vendors of proprietary platforms experimented with hy...
متن کاملthe present study is a survey os postmodern historical novel with respect to the voice of women. it argues how the recorded history is regulated by the rules of power and how women have been excluded from this discourse.ther study analysez angela carters nights at the circus as an example of postmodern historical novel to illustrate how this novel has questioned the historical narrative and how it has inserted women into history by breaking the rules of patriarchy.
the present thesis is a study of postmodern historical novel with respect to the voice of women. according to postmodern historiography,the established institution of history is one of the other manifestations of patriarchys power which has silenced women. the study analyzes angela carters nights at the circus as a postmodern historical novel which makes its female characters transgress the pat...
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ژورنال
عنوان ژورنال: Biochemistry
سال: 2013
ISSN: 0006-2960,1520-4995
DOI: 10.1021/bi401026d